What is MCF10A?
The MCF10A human breast epithelial cell line is arguably the most commonly used normal breast cell model. These cells were derived from benign proliferative breast tissue and spontaneously immortalized without defined factors. They are not tumorigenic and do not express estrogen receptor [6].
Why is cholera toxin used in cell culture?
Cholera toxin (CTX) is a protein complex secreted by the bacterium Vibrio cholerae and is responsible for the profuse, watery diarrhoea characteristic of cholera infection. It has been reported that CTX strongly stimulates colony growth from a small number of cultured human epidermal keratinocytes.
Is MCF10A cancerous?
MCF10A is a spontaneously immortalized, non-malignant breast cell line obtained from a patient with benign fibrocystic disease [32] and is the founder cell line of a progressively more aggressive family of breast cancer lines.
What does cholera toxin inhibit?
Enkephalins bind to the opioid receptors on enterocytes, which act through G proteins to inhibit the stimulation of cAMP synthesis induced by cholera toxin, thereby directly controlling ion transport.
What does hydrocortisone do in cell culture?
Overview. Hydrocortisone is a glucocorticoid hormone with anti-inflammatory and immunosuppressive effects (Kang et al.; Langhoff & Ladefoged). It is also commonly used as a supplement for endothelial, epithelial, mesenchymal, or oligodendrocyte cell culture media, as it supports growth and differentiation.
What is GM12878?
GM12878 is a lymphoblastoid cell line produced from the blood of a female donor with northern and western European ancestry by EBV transformation. It was one of the original HapMap cell lines and has been selected by the International HapMap Project for deep sequencing using the Solexa/Illumina platform.
What is Huvec cell line?
Human umbilical vein endothelial cells (HUVEC) are primary cells isolated from the vein of the umbilical cord. They are a model system for studying endothelial cell function, with applications including hypoxia, inflammation, oxidative stress, response to infection, and both normal and tumor-associated angiogenesis.
Is MCF 7 triple negative?
MCF7 and MDA-MB-231 cells represent a striking example in that they are both invasive ductal/breast carcinoma cells, yet they have many phenotypic/genotypic differences: MCF7 are hormone dependent (both estrogen and progesterone receptor positive—ER and PR), while MDA-MB-231 are triple negative.
What are tumorigenic cells?
We refer to these cells as tumorigenic cells, or cancer-initiating cells, because they consistently formed tumors, whereas other cancer cell populations were depleted of cells capable of tumor formation. We identified cell surface markers that can distinguish between these cell populations.