Is amylase hazardous?
Inhalation May be harmful if inhaled. Causes respiratory tract irritation. Skin May be harmful if absorbed throught skin. Causes skin irritation.
How do you dispose of alpha amylase?
Dispose of via a licensed waste disposal contractor. For emergency responders : If specialized clothing is required to deal with the spillage, take note of any information in Section 8 on suitable and unsuitable materials.
What are the laboratory precautions to be observed when doing amylase determination?
Since saliva is rich in amylase, it should be avoided the material and reagents contamination with saliva. Avoid using mouth to blow the water, reagent and samples away of the pipette. A decrease in the control indicates saliva contamination on the substrate.
What is amylase?
Amylase is an enzyme, or special protein, that helps you digest food. Most of your amylase is made in the pancreas and salivary glands. A small amount of amylase in your blood and urine is normal.
What precautions must be taken when working with aqueous iodine solution?
Use the lowest concentration and smallest volume possible. Wear eye protection for all but the most-dilute solutions. Handle iodine solid using forceps or wear protective gloves especially for larger amounts. Avoid breathing iodine vapour, eg, by using a fume cupboard.
What is the flammability of iodine?
Iodine is not combustible, but it is a STRONG OXIDIZER that enhances the combustion of other substances. Use water only. DO NOT USE CHEMICAL or CO2 as extinguishing agents. POISONOUS GASES ARE PRODUCED IN FIRE, including Hydrogen Iodide and other Iodine compounds.
What is alpha amylase made of?
It is the major form of amylase found in humans and other mammals. It is also present in seeds containing starch as a food reserve, and is secreted by many fungi. It is a member of glycoside hydrolase family 13. EC no….Alpha-amylase.
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Where is alpha amylase used?
starch industry
The most widespread applications of alpha amylases are in the starch industry, which is used for starch hydrolysis in the starch liquefaction process that converts starch into fructose and glucose syrups.
What is the optimum temperature for amylase?
AMYLASE has an OPTIMAL RANGE of pH and Temperature which is pH = 7 (neutral) and 37 degrees C. These are the same conditions that exist in our bodies. When an enzyme is within its Optimal Range or conditions, it will be able to catalyze reactions at its fastest rate.
How are bacterial cultures screened for amylase production?
Isolation and primary screening for amylase producers was done by using starch agar (containing 1% starch and 2% agar) plate method. Sediment samples were serially diluted up to 10-4 with sterilized 50% aged sea water and 0.1 ml the diluted samples were spread over the surface of starch agar medium.
What does alpha amylase do?
α-Amylases (E.C. 3.2. 1.1) are enzymes that catalyze the hydrolysis of the internal α-1,4-glycosidic linkages in starch, converting starch into low-molecular-weight products such as glucose, maltose, and maltotriose units (Rajagopalan and Krishnan, 2008; Gupta et al., 2003; Kandra, 2003).