What is Cytospray used for?
Cyto-Spray combines a convenient spray dispenser with a polyethylene glycol formulation to enable the rapid, high quality fixation of cytology cell spreads. Cyto-Spray covers cells with a tough, soluble film that protects cell morphology for microscopic examination.
What is cytology fixative?
Cytology Fixative covers cells with a tough, soluble film that protects cell morphology for microscopic examination. It is water and alcohol soluble, environmentally friendly and extremely economical.
Which of the following is the cytological fixatives?
Ninety-five percent (95%) ethanol is the standard cytological fixative used in many laboratories. Commercially available ethanol is expensive and not freely available in some institutions. Methanol, a tissue dehydrant, is also known to be a cytological fixative.
What is the best fixative used in Pap smear?
A 95% solution of ethyl alcohol or spray fixative is used to fix the slide. The slides should be labeled in pencil with the patient’s name AND date of birth and a properly completed Pap smear requisition should accompany the slide.
What are the different types of fixatives used in cytology?
Cytology fixatives
- DAVIDSON solution. Fixation of swab specimens.
- DAVIDSON solution, modified (Hartmann Fixative)
- DELAUNAY’s Fixative.
- ESPOSTI’s Fixative for Urine Cytology.
- Fixation Spray for Cytology.
- Fixing mixture for blood and bone marrow smears.
- Formalin-Acetone-Fixing mixture.
- MorDIFF-Quick Fixative.
What are the types of fixatives?
Popular fixative solutions
- Phosphate buffered formalin.
- Formal calcium.
- Formal saline.
- Zinc formalin (unbuffered)
- Zenker’s fixative.
- Helly’s fixative.
- B-5 fixative.
- Bouin’s solution.
What are the different types of fixatives used in histopathology and cytology?
Compound Fixatives • Microanatomical fixatives: These are used to preserve the anatomy of the tissue. Cytological fixatives: These are used to fix intracellular structures. Histochemical fixatives : These are used to demonstrate the chemical constituents of the cell.
Why is methanol a fixative?
Methanol is the simplest of the alcohols and the fixative traditionally used for blood smears to be stained with Romanowsky stains. It has also been recommended as a substitute for ethanol in Carnoy’s fluid, which is then called “methacarn”….
| Property | Data |
|---|---|
| Additive | No |
| Coagulant | Yes |
| Hardens | Yes |
| Acid dyes | Neutral |
How do you preserve a Pap smear?
Keep the preserved specimen at room temperature or refrigerated (2-8ºC). It is recommended to return the specimen to the laboratory as soon as possible after collection. Pregnant Patients: The Cervex-Brush® may be used on pregnant patients up to the 10-week mark.
What are the 3 types of specimens for Pap smear?
Acceptable specimens for the Pap Test include cervical, cervical and vaginal, endocervical, vaginal and/or vulvar scrapings or brushings.
Which fixative is generally used in cytology laboratory?
Methanol is commonly used as a fixative for blood films and 95% ethanol is used as a fixative for cytology smears but both alcohols are usually combined with other reagents when used as fixatives for tissue specimens.